The final size on disk will depend on the cluster / sector size of the underlining filesystem used by the storage device (actually, the partition) the file is in. It is rounded up to the upper nearest value multiple of the sector size of your storage (for NTFS it is 4KB by default, but can be higher / smaller).
Therefore, a single byte extra could mean a file taking up multiple kilbytes of your storage. In fact, that's the very cause for the problem of "slack space" on storage media, that is, the file wasted by unused space on the last sector of each file, a problem that is very noticieable when there are lots of really small files on a partition with a big sector size.
As to your question, there is no definite answer, and I believe it is a non-issue, unless you really need image files with such a precision in file size where a 4KB difference is significative.