I just installed a pre-RTM build of Windows 7 that I downloaded from Microsoft, so this may have changed in the RTM version, but I do not think so. (EDIT: Same thing in the released version.)
In Windows Vista, XP and older you could move icons around freely within a folder, rearranging them as you wanted them -- even though it was automatically ordering the position of them. So I could move a file starting with 'B' down below 'M'. I know this is kind of 'wrong', but is this possible in Windows 7?