I've tried playing an MP3 on two MacBooks in Google Chrome and have had no luck in playing it in the browser; I have no issues in Safari. In Chrome, the audio player controls load, but the actual audio file never loads. Looking at the HTTP requests/responses, I noticed that the MP3 returns a 200, 206, and a final 200 code in Chrome. In Safari, I just get a single 200.
An important point is that none of the MP3s served from this site load or play in Chrome. I have no problem playing MP3s from other sites.
Again, this is the MP3: http://nyc.podcast.play.it/media/d0/d0/d0/dW/dT/d7/d5/WT75_3.MP3