It won't negate the performance increases seen, but it will inevitably cause some aspects of your workflow to slow down.
The Appdata folder in your home directory will likely be the biggest introduction of perfomance loss. Applications store various amounts of information in the appdata folder, and the degree they use them will be proportional to the slowdown you see. For example, Google Chrome installs itself entirely in AppData.
How are you intending to do it? Move it and create a junction from c:\users ? Note that Chrome doesn't work properly via a junction if this is something you use.
I imagine you have decided to do this because your profile is getting so big and sucking up all your precious SSD disk space - but the reason it is getting big is because of the data that resides in it, and most of that data exists for a reason, is used by applications, and will be accessed at the slower rate the hdd delivers.
You'll still get fast boots though!
Perhaps get a copy of windirstat and get a visual view of the space taken up on your ssd - perhaps you can make more targetted decisions.