How do I correctly adjust the aperture and focus on my camera with the help of Digiflow?
When I first set up my camera I had a lot of trouble getting the focus right - I'd set the aperture first and then focus the camera. Occasionally though I'll not get it quite right - the camera would not quite be in focus or the image would either saturate or be too dark. I'd like to know how to properly focus the camera and make sure that my image will not saturate while still using the full range of the camera.
I have a camera that is already connected to the computer and I can view the image live though DigiFlow (a windows program used for image analysis by fluid dynamics experimentalists). The camera I am using senses light intensity of each pixel (but not colour) and I think is a standard type used in labs (it is not an SLR or similar, though I think this might work fine for other cameras too, so long as you were able to connect them to the computer). It has manual focus, zoom and aperture.
[Affiliation: My supervisor wrote the program, I am not affiliated in the sense that I will not have any positive repercussion to me putting up questions and answers on SO, I just know the person who made it. I can ask him any questions I like but other people can't - I wanted to record when I found out how to do something and try and build some kind of online help community, firstly among my own lab and also for people who use the program in other labs.]