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In ~/.tmux.conf, I use this bind-key
bind-key R source-file ~/.tmux.conf; display-message "~/.tmux.conf is reloaded"
but, when I use this tmux create a new session.
this is the complete file
Line 15 of your .tmux.conf, "new bash", starts a new session, with "bash" as the command to run in its first window. "new" is an alias for "new-session".
tmux a || tmux new
Required, but never shown